Canine Babesiosis (B.C) Nucleic acid testing kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the specific detection of Babesiosis (B.C) in canine EDTA whole blood and other samples.
Principle:
This kit uses the conserved gene fragment of Canine Babesiosis (B.C) to design a specific probe. The probe can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of the Taq enzyme will 5 The '-end fluorescent group is cleaved from the probe, making it free in the reaction system, thereby detaching from the 3'-end quenching group and emitting fluorescence, which is detected by a fluorescence PCR instrument to realize the detection of Canine Babesiosis (B.C) in a fully closed reaction system. Detection of Beth worm nucleic acid.
Main components:
| Component |
Specification |
| PCR reaction liquid |
8 tubes |
| Positive control |
1 tube |
| Negative control |
1 tube |
Note: components in different batches of kits should not be interchangeable.
Sample requirements:
1. Sample type
Canine EDTA whole blood etc.
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Method calibration:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step |
Cycle number |
Temperature |
Time |
| 1 |
1 |
95℃ |
3min |
| 2 |
45 |
95℃ |
10s |
| 60℃ |
30s collect fluorescent |
FAM is selected for detection channels.
Positive judgment value or reference interval
| Results |
Ct Value |
| Positive |
Ct≤40 |
| Negative |
No Ct value or Ct value≥45 |
| Gray area |
40＜Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Product Tags:
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Canine Babesiosis Pcr Kit EDTA Nucleic Acid Testing Kit PCR Fluorescent Images
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